Improved method for measurement of delta-aminolevulinic acid dehydratase activity of human erythrocytes.
نویسندگان
چکیده
We have evaluated and modified a current method for estimating 8-aminoIevulinic acid dehydratase (5-aminolaevulinate hydro-lyase, EC 4.2.1.24) activity in blood, so as to adequately control the pH of the enzyme-substrate solution at the optimum throughout the incubation period. This has improved the sensitivity and reliability of the assay. Also, N-ethylmaleimide has been substituted for a potentially hazardous mercury salt, used to remove sulfhydryl groups before color development with a modified Ehrlich’s reagent.
منابع مشابه
Lead toxicity in the pregnant rat. II. Effects of low-level lead on delta-aminolevulinic acid dehydratase activity in maternal and fetal blood or tissue.
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عنوان ژورنال:
- Clinical chemistry
دوره 17 10 شماره
صفحات -
تاریخ انتشار 1971